Larvicidal, ovicidal and repellent activity of Nerium oleander leaves against Japanese encephalitis vectors

  • Kokati Venkata Bhaskara Rao Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu - 632014, India
  • Loganathan Karthik Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu - 632014, India
  • Arivarasan Vishnu Kirthi Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509, Vellore, Tamil Nadu, India
  • Abdul Abdul Rahuman Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509, Vellore, Tamil Nadu, India
  • Gaurav Kumar Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, 144411, Punjab, India

Abstract

The objective of this study was to assess the mosquito controlling potential of aqueous extract of Nerium oleander leaves against the primary and secondary vectors of Japanese encephalitis (Culex tritaeniorhynchus and Cx. gelidus). Phytochemical screening of N. oleander leaves exhibited the presence of saponins, proteins, flavonoids, alkaloids, tannins, phenolic compounds, carbohydrates, glycosides, phytosterols, oil and fats. Extract treatment (1000 ppm) resulted in 100% mortality against 4th instar larvae of Cx. tritaeniorhynchus and Cx. gelidus. Extract treatment (1000 ppm) resulted in mild ovicidal activity (10±1.22% hatching) against Cx. tritaeniorhynchus, whereas, 100% ovicidal property against Cx. gelidus eggs. Extract treatment (1000 ppm) also delivered comprehensive protection from mosquito bite for 240 and 120 minutes against Cx. tritaeniorhynchus and Cx. gelidus, respectively. The results of this study suggest the mosquito controlling potential of N. oleander leaves and provides a potential option for the researchers to prepare natural, safe, and eco-friendly insecticide.                         

Article Level Metrics

Download data is not yet available.
Published
2017-06-25
How to Cite
VENKATA BHASKARA RAO, Kokati et al. Larvicidal, ovicidal and repellent activity of Nerium oleander leaves against Japanese encephalitis vectors. International Journal of Research in Pharmaceutical Sciences, [S.l.], v. 8, n. 2, p. 157-162, june 2017. ISSN 0975-7538. Available at: <https://www.pharmascope.org/index.php/ijrps/article/view/894>. Date accessed: 22 nov. 2017.
Section
Research Articles