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A simple, fast and precise quantitative high performance thin-layer chromatographic method has been developed for quantitative estimation of α-mangostin in young pericarp, ripe pericarp and bark extract of Garcinia mangostana L. (Hypericaceae). Determination was performed by using TLC plates with F254 silica gel as stationary phase and chloroform-methanol (10: 0,1) as mobile phase. 2 μL of extract spotted on TLC plate, then eluted and analyzed by using densitometry. Concentration of α-mangostin was determined by using calibration curve from some standard concentrations of α-mangostin. This study performed that the highest concentration of α-mangostin was in ripe pericarp (15,85%). Meanwhile, concentration of α-mangostin in young pericarp was 4,19% and 3,88% in bark extract.
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