Plant growth-promoting and antagonistic endophytic bacteria from the medicinal plant Tinospora cordifolia stem
Endophytic bacteria residing within the plant tissues are poorly investigated microorganisms. A total of 35 endophytic bacteria isolated from Tinospora cordifolia stem tissue samples collected from 3 different districts of Punjab, India were screened for multiple plant growth-promoting attributes of phosphate solubilization, IAA-like auxin production, siderophore production, ACC-deaminase activity, hydrogen cyanide and ammonia production. Out of these bacteria, 11 showing multiple plant growth-promoting activities were screened for anatagonism against phytopathogens Fusarium moniliforme, Alternaria alternata and Curvularia lunata. One isolate TCA2 showed broad-spectrum activity with 38% growth inhibition in Alternaria alternata, 35% inhibition in Fusarium moniliforme and 25% inhibition in Curvularia lunata was identified as Pseudomonas sp. based on 16s rRNA gene sequencing. The maize plants inoculated with the bacterial isolate TCA2 showed 33.1%, 31.6%, and 38.4% increase in root length, shoot length and dry weight, respectively, over uninoculated control in pots under natural conditions. This is the first report on the plant growth-promoting endophytic bacteria from Tinospora cordifolia. In the present studies, Pseudomonas sp. TCA2 with multiple plant growth-promoting activities has been selected as a suitable candidate for the development of microbial inoculants.
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