Isolation of multidrug-resistant bacteria from the hospital environment

  • Mahavir Joshi University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413 India
  • Sukhminderjit Kaur University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413 India
  • Bhavkiara Shergill University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413 India
  • Sonali Sood University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413 India
  • Mishra Tulika School Of Medicine, Xavier University, Santa Helenastraat #23, Oranjestad, Aruba, Dutch Caribbean

Abstract

Antibiotics are used to cure the illness caused by pathogenic microbes. The resistance towards such antibiotics is becoming a serious concern in the present time. The frequency of drug resistance is increasing in hospitals. This aptitude to develop resistance against antibiotics has become a serious threat to the patients that are already hospitalized, making them more prone to infections and increased complications of already existing medical conditions. It can also lead to a high mortality rate in hospitals. The present work is designed to isolate microbes from the hospital environment to check the sensitivity against various antibiotics. For the isolation, aerosol-based air samples were taken by exposing sterile Petri plate at the OPD and general ward for about 10 minutes after that the plates were taken to the lab and incubated at 37oc for 24 hours. Pure cultures were obtained by sub culturing the isolates onto fresh sterile nutrient agar plates. The clinical isolates were tested for antibiotic sensitivity test by using Dodeca G- V Plus disk (Himedia). The isolates were identified on the basis of microscopic and VITEK 2 based identification. Total of four bacteria Kocuria kristinae, Sphingomonas paucimobilis, Pantoea spp. and Staphylococcus vitulinus were isolated that showed variation in antibiotic-resistant pattern.

Keywords: Antibiotic resistance microorganisms, Kocuria kristinae, Pantoea spp, Sphingomonas pauci-mobilis, Staphylococcus vitulinus, VITEK 2

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Published
2019-04-14
Citations
How to Cite
Mahavir Joshi, Sukhminderjit Kaur, Bhavkiara Shergill, Sonali Sood, & Mishra Tulika. (2019). Isolation of multidrug-resistant bacteria from the hospital environment. International Journal of Research in Pharmaceutical Sciences, 10(2), 990-996. https://doi.org/10.26452/ijrps.v10i2.370
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Original Articles
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