Anti-oxidant, Cytotoxic and Apoptotic studies of root bark assisted Silver Nanoparticles from Aphanamixis polystachya

  • Rajashree Gude Department of Pharmaceutics, Goa College of Pharmacy, 18th June Road, Panaji Goa, India
  • Arun B. Joshi Department of Pharmacognosy, Goa College of Pharmacy, 18th June Road, Panaji Goa, India
  • Anant Bhandarkar Department of Pharmacognosy, Goa College of Pharmacy, 18th June Road, Panaji Goa, India

Abstract

The aim of the present investigations was to fabricate and prepare silver nanoparticles from the root bark of Aphanamixis polystachya available in Western Ghats of India and also to assess their antioxidant, cytotoxic and apoptotic activity. The root bark extract of the plant, A. polystachya was used for the green synthesis of silver nanoparticles (AgNP). The AgNP were characterized with the help of UV-Vis Spectrophotometry, Fourier-transform infrared spectroscopy (FTIR), Energy Dispersive X-Ray Analysis (EDAX), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray Diffraction (XRD)and Thermogravimetric analysis. The cytotoxic and apoptotic effects were studied using methanolic extract and AgNP of A. polystachya on human breast cancer cells (MCF-7 and MDA-MB-231). Silver nanosuspension was formulated, and in vitro, antioxidant activity by DPPH assay and Nitric Oxide assay were assessed on a methanolic extract of the root bark and silver nanosuspension of A. polystachya. The percentage cell growth on MCF-7 cells was found to be 39.8, 21.6, 18.0 and 15.6 at 10, 20, 40 and 80µg/ml respectively. In the case of MDA-MB231 cells, the percentage of cell growth was found to be 99.2, 80.0, 67.2 and 54.6 at 10, 20, 40 and 80 µg/ml. Growth inhibition (GI50) of <10µg and >80µg was observed in MCF-7 and MDA-MB231 cell lines. A. polystachya silver nanosuspension significantly enhanced the antiradical potential against nitric oxide scavenging assay (IC50 4.7µg/ml) as compared with methanolic extract of A. polystachya. The formulation of silver nanosuspension of A. polystachya significantly enhanced the antiradical potential as compared with the methanolic extract of root bark of A. polystachya.

Keywords: Apoptosis, Silver nanosuspension, DPPH, Nitric Oxide

Downloads

Download data is not yet available.
Published
2019-04-14
Citations
How to Cite
Rajashree Gude, Arun B. Joshi, & Anant Bhandarkar. (2019). Anti-oxidant, Cytotoxic and Apoptotic studies of root bark assisted Silver Nanoparticles from Aphanamixis polystachya. International Journal of Research in Pharmaceutical Sciences, 10(2), 943-950. https://doi.org/10.26452/ijrps.v10i2.365
Section
Original Articles
Article Level Metrix
Abstract Views: 171
PDF Downloaded: 87