Analysis of Strychnine and Brucine in hydro ethanolic extract of Strychnos Nux-Vomica leaves by using HPTLC
Strychnos Nux-Vomica is a remedial plant used in herbal medicine and medicinal plants is having a curative history and are still helping healthiness requirements of a large population in the world. Therapeutic plants are used to cure various diseases, these are the phytochemicals extracted from plants. Strychnos-nux-vomica is having Strychnine and Brucine with biological properties. However, qualitative and quantification of the compounds in plant extract is determined by using chromatography techniques. The method HPTLC is used in identification, standardization and quantification of bioactive Phytoconstituent in plant extract and these extracts helpful in medications. Strychnos Nux-vomica leaves are extracted with 50% Ethanol in a water and centrifuged at 3000 rpm for 5min for analysis. 3 µl of Sample S and 2 µl of standard solution were loaded in CAMAG LINOMAT 5 instrument and scanning done at UV 254nm. The Mobile phase is used ratio of Toluene – Ethyl acetate – Diethyl amine (7:2:1) and Dragendorff's reagent is used as spraying for spot development. HPTLC chromatograms shows the Strychnine, Brucine and eight unknown compounds. Strychnine, Brucine Rf values are 0.50, 0.33 and values of the area is 16667.7 and 13826.4.