Extracellular secretion recombinant of human epidermal growth factor (hEGF) using pectate lyase B (PelB) signal peptide in escherichia coli BL21(DE3)

  • Sriwidodo . Pharmaceutic & Pharmaceutical Technology Department, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia
  • Iman P. Maksum Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia
  • N Riswanto Pharmaceutic & Pharmaceutical Technology Department, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia
  • Tina Rostinawati Pharmaceutic & Pharmaceutical Technology Department, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia
  • Toto Subroto Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia

Abstract

The extracellular expression of high valued therapeutic proteins such as Human Epidermal Growth Factor (hEGF) from Escherichia coli has become a challenge yet give some advantages. It ensures the correct folding of recombinant hEGF (rhEGF) so the therapeutic activity and immunogenicity profile will be similar with the native hEGF. In addition, extracellular expression eliminates the host cell disruption and simplifies the purification process, although other study has shown the protein yield from E. coli extracellular expression is lower than the intracellular expression. Various factors such as codon usage, inducer concentration, induction time, and harvest time can be optimized to increase the rhEGF secretion. The aim of this research is to express rhEGF extracellularly from E. coli BL21 (DE3). The expression system was supported by optimized codon usage to E. coli codon preference, pectate lyase B (PelB) signal peptide in the pD881-PelB expression vector, and L-rhamnose as an inducer. Growth curve of E. coli was made to determine the L-rhamnose induction time. Induction was performed with 4mM L-Rhamnose at OD600 0.7. rhEGF in the soluble fraction, periplasmic extraction, and culture medium was characterized by tricine SDS-PAGE. Quantification of rhEGF concentration was performed by ELISA. The codon optimization showed that the Codon Adaptive Index (CAI) of rhEGF gene was 1, GC percentage was 50.93% and relative adapts was 100% after codon usage optimization. SDS-PAGE showed 6.2 kDa band of rhEGF band from soluble fraction, periplasmic extraction (after 18 hour induction), and culture medium. The quantification of rhHEGF by ELISA showed the rhEGF concentration was 310.8 µg/mL.                         

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Published
2017-03-24
How to Cite
., Sriwidodo et al. Extracellular secretion recombinant of human epidermal growth factor (hEGF) using pectate lyase B (PelB) signal peptide in escherichia coli BL21(DE3). International Journal of Research in Pharmaceutical Sciences, [S.l.], v. 8, n. 1, p. 33-40, mar. 2017. ISSN 0975-7538. Available at: <https://www.pharmascope.org/index.php/ijrps/article/view/311>. Date accessed: 24 sep. 2017.
Section
Research Articles
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