Comparative studies on phytochemistry, antioxidant and antibacterial activity of direct and sequential extracts of chromolaena odorata leaves

  • Udaya Prakash N K Deparment of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai 600117, India
  • Sriraman V Deparment of Chemistry, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai 600117, India
  • Devika V Deparment of Chemistry, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai 600117, India
  • Peneena V Deparment of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai 600117, India
  • Sripriya N R & D, Marina Labs, Nerkundram, Chennai 600107, India
  • Bhuvaneswari S Department of Botany, Bharathi Women’s College, Broadway, Chennai 600108, India
  • Sampathkumar B Deparment of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai 600117, India

Abstract

Phytochemistry, antioxidant and antibacterial activities of different solvent extracts of Chromolaena odorata leaves obtained by direct and sequential extraction were compared in this study. Antibacterial evaluation of the extracts was performed through a disc diffusion method. The results revealed that the solvents, Petroleum ether and Ethyl acetate showed the presence of a majority of the phytochemicals, Chloroform extract yielded maximum TPC and TAA and ethyl acetate yielded higher TFC when compared to other solvents. Ethanol and chloroform extracts showed lower concentrations of IC50 against DPPH and NO radicals respectively. Acetone recorded the maximum zone of inhibition against Staphylococcus aureus and Pseudomonas aeruginosa; ethyl acetate against Bacillus subtilis and Chloroform against Streptococcus mutans. Among the solvents studied for phytochemical content and bioactivities, ethanol showed a higher level of phytochemical extraction and better bioactivities. As a direct solvent extraction of C. odorata showed better efficiency when compared with sequential extraction, the study strongly recommends the use of a direct extraction method.

Keywords: Antibacterial activity, Antioxidant activity, Chromolaena odorata, Direct extraction, Polarity, Sequential extraction

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Published
2019-04-15
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How to Cite
Udaya Prakash N K, Sriraman V, Devika V, Peneena V, Sripriya N, Bhuvaneswari S, & Sampathkumar B. (2019). Comparative studies on phytochemistry, antioxidant and antibacterial activity of direct and sequential extracts of chromolaena odorata leaves. International Journal of Research in Pharmaceutical Sciences, 10(2), 914-921. https://doi.org/10.26452/ijrps.v10i2.275
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Original Articles
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