Determination of enterotoxin genes in Bacillus cereus from raw milk using multiplex polymerase chain reaction technique

  • Nisreen Jawad K School of Biosciences and Biotechnology, Faculty of Science and Technology Universiti Kebangsaan, Bangi, Selangor 43600 Malaysia
  • WS Aqma School of Biosciences and Biotechnology, Faculty of Science and Technology Universiti Kebangsaan, Bangi, Selangor 43600 Malaysia
  • Fathul K School of Environmental Sciences Faculty of Science and Technology Universiti Kebangsaan, Bangi, Selangor 43600 Malaysia
  • Heng Lee Y School of Chemical Sciences and Food Technology, Faculty of Science and Technology Universiti Kebangsaan, Bangi, Selangor 43600 Malaysia
  • Asmat A School of Biosciences and Biotechnology, Faculty of Science and Technology Universiti Kebangsaan, Bangi, Selangor 43600 Malaysia

Abstract

A Gram-positive bacterium, which can usually be found in cultivating plants and soil, causing it commonly universal is regarded as Bacillus cereus. It is said that most strains present in B. cereus is specifically called the primary cause of food poisoning illnesses which is characterised by either emetic or diarrheal syndrome, the most essential purpose of the current research was, therefore, to isolate and identify the enterotoxin genes in B. cereus through using a multiplex polymerase chain reaction (mPCR)technique. To achieve this aim, thus B.cereus was isolated from raw milk then cultured on mannitol egg yolk polymyxin agar and chromogenic B. cereus agar. The samples were then examined using biochemical investigation then established using partial sequencing of 16SrDNA. Among 40 samples, 20 of them were found to contain hblC and hblD, a hemolytic enterotoxin (HBL) complex encoding genes. The total mean of contamination samples in the B. cereus was proved to be higher (1.4×105 – 3.2×105 CFU/mL) than the acceptable detection level of B. cereus in dairy products ~103 CFU/mL. The isolates (10%) were shown to express hblC gene whereas the isolates (20%) were shown to express the hblD gene. The findings suggested that the detected enterotoxin genes of B. cereus which were found in raw milk may create a potential danger to the health of the public.

Keywords: Bacillus cereus, Enterotoxin genes, Multiplex polymerase, chain reaction, Raw milk

Downloads

Download data is not yet available.
Published
2019-01-05
Citations
How to Cite
Nisreen Jawad K, WS Aqma, Fathul K, Heng Lee Y, & Asmat A. (2019). Determination of enterotoxin genes in Bacillus cereus from raw milk using multiplex polymerase chain reaction technique. International Journal of Research in Pharmaceutical Sciences, 10(1), 645-649. Retrieved from https://www.pharmascope.org/index.php/ijrps/article/view/118
Section
Original Articles
Article Level Metrix
Abstract Views: 16
PDF Downloaded: 16